Medimabs/Leu-脑啡肽小鼠单克隆抗体（NOC1）/200µL/MM-0006-P
商品编号：
MM-0006-P
品牌：
Medimabs inc
市场价：
¥7100.00
美元价：
5680.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Target:
Leu-enkephalin
Target background:
Enkephalins (penta-peptides) have been discovered as regulators of nerve impulses involving pain in the brain. It has been shown that these peptides act as natural analgesics (pain-killers) and their action mimics that of morphine and other opiates. At present, it is thought that the morphine-like effects are due to aromatic side chains on phenylanine and tyrosine which mimic a similar structure on morphine. Research suggests that it does not make much difference whether the enkephalin contains methionine or leucine at the acid end of the peptide for its action.
Target alias:
Enkephalin, endorphins, endorphin, Met5-enkephalin, Leu5-enkephalin, Met-enkephalin, Leu-enkephalin, anti-Enkephalin, anti-endorphins, anti-endorphin, anti-Met5-enkephalin, anti-Leu5-enkephalin, anti-Met-enkephalin, anti-Leu-enkephalin
Immunogen:
pentapeptide ( Tyr-Gly-Gly-Phe-Leu)
Specificity: A sensitive antibody against enkephalinergic fibrilar networks in the globus pallidus, N. accumbens, septum, hypothalamus, thalamus, amygdala, N. interstitialis of the stria terminalis and brain stem nuclei. This antibody does not distinguish between Met5-enkephalin and Leu5-enkephalin by radioimmunoassay or by immunocytochemistry. It is recommended for localizing enkephalinergic sites by immunohistochemistry. It does not bind to beta-endorphin or dynorphin-containing areas. It exhibits approximately. 40% cross reactivity with c-terminal extended Met-enkephalin hexapeptides and 7% cross reactivity with the extended heptapeptide (-Arg-Phe-0H), but does not recognize other endogenous peptides. All wellestablished enkephalin immunoreactive sites are recognized by this antibody in immunohistochemistry (IHC) and electron microscopy immunocytochemistry (ICC). Specific for: rat and human
Clone ID:
NOC1
Isotype:
IgG1
Preservative:
None
Format:
Lyophilized tissue culture supernatant
Recommend starting dilution:
If reconstituted with deionized water in 200 µL: IHC / ICC 1:200 - 1:800. Optimal dilution has to be determined by the user.
Limitations:
Research Use Only
References:
1.-Bernácer J - Cholinergic interneurons are differentially distributed in the human striatum.
2.-Andersson LI - Mimics of the binding sites of opioid receptors obtained by molecular imprinting of enkephalin and morphine.
3.-Cuello AC - Characterization and immunocytochemical application of monoclonal antibodies against enkephalins.
4.-Ma W - Substance P and enkephalin immunoreactivities in axonal boutons presynaptic to physiologically identified dorsal horn neurons. An ultrastructural m...
5.-Garzón M and Pickel VM - Ultrastructural localization of enkephalin and mu-opioid receptors in the rat ventral tegmental area.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
品牌介绍
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