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Medimabs/SWE1 Rat Monoclonal Antibody (15F2)/100 µg/MM-0274-P
Medimabs/SWE1 Rat Monoclonal Antibody (15F2)/100 µg/MM-0274-P
商品编号：
MM-0274-P
品牌：
Medimabs inc
市场价：
¥7700.00
美元价：
6160.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Target:
SWE1
Target background:
Homologue of Wee1, Swe1 is a checkpoint kinase found in the budding yeast Saccharomyces cerevisiae. Swe1 imposes a delay between the G2 and M phases of the cell cycle, inhibiting entry into mitosis by inhibiting the activity of Cdc28 through phosphorylation at tyrosine residue 19. Swe-1 is negatively regulated by the proteins Hsl1 and Hsl7, and a complex of the three proteins is localized at the bud neck of the yeast. Swe1 is also thought to be SUMOylated, and ubiquitination of Swe1 controlled by Dma1 and Dma2 ubiquitin ligases. The kinase Swe1 controls mitotic spindle elongation and is thought to regulate the filamentous growth and differentiation of S. cerevisiae.
Target alias:
Mitosis inhibitor protein kinase SWE1, Wee1 homolog
Immunogen:
Recombinant Swe1 protein
Specificity: The antibody recognizes the Swe1 protein in yeast synchronized in S phase, and is negative for yeast synchronized in G1 phase.
Clone ID:
15F2
Isotype:
IgG2a kappa
Preservative:
None
Format:
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100 µl: WB: 1:250. For western blots, it is strongly recommended that MM-0273 anti-Swe1 (18D9G8A1) and MM-0274 anti-Swe1 (15F2) be used in combination at a 1:250 dilution for efficient detection of Swe1. Optimal dilution has to be determined by the user.
Limitations:
Research Use Only
References:
1.-Ratsima H - Centrosome-Dependent Bypass of the DNA Damage Checkpoint by the Polo Kinase Cdc5.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
品牌介绍
Medimabs让我们的专家团队为您定制抗体的开发确定最佳方法：肽，蛋白质或DNA免疫。受益于他们的专业战略建议，可靠的抗原设计和快速合成抗体开发中最关键的一步是抗原策略和设计。为了使您的项目成功的机会最大化，我们将深入分析目标的结构，并找到最佳的选择以获得适合您的应用需求的抗体。整个项目可以根据您的最终应用进行定制（流式细胞仪，治疗，蛋白质印迹，免疫荧光，ELISA，免疫沉淀等）。我们可以获得广泛的免疫原，并且每种免疫原都有其优势。 合成肽蛋白质（细菌，酵母，昆虫或哺乳动物细胞表达模型） DNA免疫让我们指导您，为您的项目设计最佳抗原。
