Medimabs/HIV-1 (V3 (gp120)) Human Monoclonal Antibody (2558)/100 µg/MM-0254-P
商品编号：
MM-0254-P
品牌：
Medimabs inc
市场价：
¥10100.00
美元价：
8080.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Target:
HIV-1 (V3 (gp120))
Target background:
Of the two types of Human immunodeficiency virus (HIV) which have been characterized, HIV-1 is more infective and is prevalent globally. The HIV virion is encapsulated by a viral envelope that contains complexes of surface proteins, which are important for the virus to attach and fuse to infect target cells. A precursor molecule known as gp160 (glycoprotein 160) is cleaved by proteases into gp120 (glycoprotein 120) and gp41 (glycoprotein 41), glycoproteins that make up the envelope proteins of HIV. The gp120 protein is composed of five variable regions (V1-V5) which often form loop structures, and five constant regions (C1-C5). The third variable loop (V3 loop) of gp120 interacts with cytokine receptors to mediate the entry of the virus into cells. This antibody, clone 2558, is directed against the V3 loop of gp120 and has been shown to neutralize HIV-1 primary isolates.
Immunogen:
This antibody was generated from Cameroonian HIV-1, CRF02 AG-infected individuals by a method based on the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) followed by fusion with heteromyeloma cells. The antibody recognizes the epitope YNKRKRIHI.
Specificity: The antibody recognizes the V3 loop of the HIV-1 protein gp120.
Clone ID:
2558
Isotype:
IgG1 lambda
Preservative:
None
Format:
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
Reconstitute with deionized water. Optimal dilution has to be determined by the user.
Limitations:
Research Use Only
References:
1.-Andrabi R - Cross-neutralizing activity of human anti-V3 monoclonal antibodies derived from non-B clade HIV-1 infected individuals.
2.-Gorny MK - Human anti-V3 HIV-1 monoclonal antibodies encoded by the VH5-51/VL lambda genes define a conserved antigenic structure.
3.-Gorny MK - Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1.
4.-Gorny MK - The v3 loop is accessible on the surface of most human immunodeficiency virus type 1 primary isolates and serves as a neutralization epitope.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
品牌介绍
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