Medimabs/DICER Rabbit Polyclonal Antibody/50 µg/MM-0229-P
商品编号：
MM-0229-P
品牌：
Medimabs inc
市场价：
¥6100.00
美元价：
4880.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Target:
DICER
Target background:
Dicer is an endoribonuclease which has two RNase III domains and one PAZ domain and belongs to the highly conserved family of dsRNA-specific endoribonucleases RNase III family. Dicer plays important roles in RNA processing, post-transcriptional gene expression control and defense against viral infection. Synthesis of proteins can be blocked by RNA in a process known as RNA interference and for this process to happen; three important events have to occur. First, DICER processes dsRNA into smaller fragments known as short interfering RNAs (siRNAs) and microRNAs (miRNAs). Second, a dsRNA binding protein recruits a DICER-siRNA complex to Argonaute2 (Ago2), which is an endonuclease capable of degrading messenger RNA (mRNA). Third, Dicer facilitates the formation of a large multiprotein complex called RISC (RNA-Induced Silencing Complex). RISC can seek out and capture messenger RNA (mRNA) molecules with a base sequence complementary to that of its siRNA or miRNA. This serves to either destroy the genetic message carried by the mRNA outright or else block the subsequent synthesis of a protein.
Specificity: The antibody recognizes isoforms 1 and 2 of DICER.
Clone ID:
---
Preservative:
None
Format:
Lyophilized immunogen affinity purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 50 µl: WB: 1:1000 – 1:3000. Optimal dilution has to be determined by the user.
Limitations:
Research Use Only
References:
1.-Daniels SM - HIV-1 RRE RNA acts as an RNA silencing suppressor by competing with TRBP-bound siRNAs.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
品牌介绍
Medimabs让我们的专家团队为您定制抗体的开发确定最佳方法：肽，蛋白质或DNA免疫。受益于他们的专业战略建议，可靠的抗原设计和快速合成抗体开发中最关键的一步是抗原策略和设计。为了使您的项目成功的机会最大化，我们将深入分析目标的结构，并找到最佳的选择以获得适合您的应用需求的抗体。整个项目可以根据您的最终应用进行定制（流式细胞仪，治疗，蛋白质印迹，免疫荧光，ELISA，免疫沉淀等）。我们可以获得广泛的免疫原，并且每种免疫原都有其优势。 合成肽蛋白质（细菌，酵母，昆虫或哺乳动物细胞表达模型） DNA免疫让我们指导您，为您的项目设计最佳抗原。
