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Medimabs/Misfolded SOD1 Mouse Monoclonal Antibody (C4F6)/200 µL/MM-0070-2-P
Medimabs/Misfolded SOD1 Mouse Monoclonal Antibody (C4F6)/200 µL/MM-0070-2-P
商品编号：
MM-0070-2-P
品牌：
Medimabs inc
市场价：
¥9900.00
美元价：
7920.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Target:
Misfolded SOD1
Target background:
Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic and mitochondrial intermembrane space protein. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. Mutations in SOD1 cause familial amyotrophic lateral sclerosis type 1 (ALS1). These mutations have been linked to accumulation of harmful superoxide radicals, promotion of apoptosis, formation of aggregates of misfolded superoxide dismutase which are toxic and the continued stimulation of nerve cells that causes them to burn out and die.
Target alias:
Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSOD1, SOD1
Immunogen:
Recombinant human SOD1 G93A APO
Specificity:
The antibody recognizes misfolded forms of mutant human SOD1 protein. IF: WT, G93A, G85R, G127X, G37R and A4V; WB (denatured): WT, G93A, D90A, G37R and A4V; WB (native): G93A; IHC: WT, G93A and G37R.
Clone ID:
C4F6
Isotype:
IgG2a
Preservative:
None
Format:
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 200 µL: IF 1:50 to 1:500; WB 1:250; IP 6 µL / 40 µL (Protein G beads / 300 µg of protein lysate). Optimal dilution has to be determined by the user.
Limitations:
Research Use Only
References:
1.-Da Cruz S - Misfolded SOD1 is not a primary component of sporadic ALS.
2.-Pickles S - ALS-linked misfolded SOD1 species have divergent impacts on mitochondria.
3.-Ayers JI - Conformational specificity of the C4F6 SOD1 antibody; low frequency of reactivity in sporadic ALS cases.
4.-Gros-Louis F - Intracerebroventricular infusion of monoclonal antibody or its derived Fab fragment against misfolded forms...
5.-Pickles S - Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1.
6.-Roberts BR - Oral treatment with Cu(II)(atsm) increases mutant SOD1 in vivo but protects motor neurons and improves the...
7.-Brown HH and Borchelt DR - Analysis of mutant SOD1 electrophoretic mobility by Blue Native gel electrophoresis; evidence...
8.-Bosco DA - Wild-type and mutant SOD1 share an aberrant conformation and a common pathogenic pathway in ALS.
9.-Chhangani D - Mahogunin ring finger 1 confers cytoprotection against mutant SOD1 aggresomes and is defective in an ALS...
10.-Jouroukhin Y - NAP (davunetide) modifies disease progression in a mouse model of severe neurodegeneration: protection...
11.-Nizzardo M - Morpholino-mediated SOD1 reduction ameliorates an amyotrophic lateral sclerosis disease phenotype.
12.-Patel P - Adeno-associated virus-mediated delivery of a recombinant single-chain antibody against misfolded superoxide...
13.-Redler RL - Non-native soluble oligomers of Cu/Zn superoxide dismutase (SOD1) contain a conformational epitope linked...
14.-Sábado J - Adverse effects of a SOD1-peptide immunotherapy on SOD1 G93A mouse slow model of amyotrophic lateral sclerosis.
15.-Xu G - Substantially elevating the levels of αB-crystallin in spinal motor neurons of mutant SOD1 mice does not...
16.-Xu G - Direct and indirect mechanisms for wild-type SOD1 to enhance the toxicity of mutant SOD1 in bigenic transgenic mice.
17.-Okamoto Y - Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic...
18.-Urushitani M - Therapeutic effects of immunization with mutant superoxide dismutase in mice models of amyotrophic...
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
品牌介绍
Medimabs让我们的专家团队为您定制抗体的开发确定最佳方法：肽，蛋白质或DNA免疫。受益于他们的专业战略建议，可靠的抗原设计和快速合成抗体开发中最关键的一步是抗原策略和设计。为了使您的项目成功的机会最大化，我们将深入分析目标的结构，并找到最佳的选择以获得适合您的应用需求的抗体。整个项目可以根据您的最终应用进行定制（流式细胞仪，治疗，蛋白质印迹，免疫荧光，ELISA，免疫沉淀等）。我们可以获得广泛的免疫原，并且每种免疫原都有其优势。 合成肽蛋白质（细菌，酵母，昆虫或哺乳动物细胞表达模型） DNA免疫让我们指导您，为您的项目设计最佳抗原。
