Medimabs/Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody/200 µL/MM-0033
商品编号：
MM-0033
品牌：
Medimabs inc
市场价：
¥7100.00
美元价：
5680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Target:
Clathrin Light Chain (CLCa / CLCb)
Target background:
Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles.Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC).CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons.Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.
Target alias:
Clathrin light chain A, Lca
Immunogen:
peptide in N term
Specificity: The antibody recognizes the N-terminal portion of Clathrin Light Chain
Clone ID:
---
Preservative:
None
Format:
Lyophilized immunogen affinity purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 200 µL: IF 1:100 to 1:500; WB 1:1000-1:2000. Optimal dilution has to be determined by the user.
Limitations:
Research Use Only
References:
1.-Allaire PD - Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis.
2.-Ybe JA - Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges.
3.-Girard M - Non-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles...
4.-Hayashi M - Cell- and stimulus-dependent heterogeneity of synaptic vesicle endocytic recycling mechanisms revealed by studies of dynamin 1-null neurons.
5.-Poupon V - Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
品牌介绍
Medimabs让我们的专家团队为您定制抗体的开发确定最佳方法：肽，蛋白质或DNA免疫。受益于他们的专业战略建议，可靠的抗原设计和快速合成抗体开发中最关键的一步是抗原策略和设计。为了使您的项目成功的机会最大化，我们将深入分析目标的结构，并找到最佳的选择以获得适合您的应用需求的抗体。整个项目可以根据您的最终应用进行定制（流式细胞仪，治疗，蛋白质印迹，免疫荧光，ELISA，免疫沉淀等）。我们可以获得广泛的免疫原，并且每种免疫原都有其优势。 合成肽蛋白质（细菌，酵母，昆虫或哺乳动物细胞表达模型） DNA免疫让我们指导您，为您的项目设计最佳抗原。
